Cytotoxic Activity Test of Kepel Leaf Extract and Fraction (Stelechocarpus burahol Hook. f. and Thomson) Against T47D Breast Cancer Cells and P53 and BCL-2 Protein Expression

ratna sari dewi; Titik Sunarni; Nuraini Harmastuti

doi:10.55927/eajmr.v4i11.365

Authors

ratna sari dewi Setia Budi University
Titik Sunarni Setia Budi University
Nuraini Harmastuti Setia Budi University

DOI:

https://doi.org/10.55927/eajmr.v4i11.365

Keywords:

Kepel Leaf Cytotoxic, p53 and bcl-2 expression, T47D breast cancer cells

Abstract

This study aims to determine the cytotoxic activity of ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction of kepel leaves (Stelechocarpus burahol Hook. Data were analyzed using anova test. The results showed that ethanol extract has cytotoxic activity against T47D breast cancer cells with IC50 value of 53.766 µg/ml, while the results of n-hexane fraction, ethyl acetate fraction and water fraction have IC50 values of 178.184 µg/ml, 52.766 µg/ml and 120.754 µg/ml. The most active fraction according to the IC50 value is the ethyl acetate fraction, although there is no significant difference with the ethanol extract. Extracts and ethyl acetate fractions have selectivity values against T47D breast cancer cells. Protein expression results that the extract and ethyl acetate fraction were able to increase p53 protein expression with a value of 50.743 ± 4.65 µg/ml and 49.869 ± 1.67 µg/ml and were able to inhibit bcl-2 protein expression with a value of 53.428 ± 0.822 µg/ml and 52.511 ± 0.437 µg/ml.

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