Antibacterial Activity of Subfraction of Ethanol Extract of Kebo Rubber Leaves (Ficus Elastica Roxb. ex Hornem) Escherichia Coli Bacteria

Adniana Yeliza; Ismi Rahmawati; Dian  Marlina

doi:10.55927/eajmr.v4i2.9

Authors

Adniana Yeliza Faculty of Pharmacy, Universitas Setia Budi, Mojosongo, Jebres District, Surakarta
Ismi Rahmawati Faculty of Pharmacy, Universitas Setia Budi, Mojosongo, Jebres District, Surakarta
Dian Marlina Faculty of Pharmacy, Universitas Setia Budi, Mojosongo, Jebres District, Surakarta

DOI:

https://doi.org/10.55927/eajmr.v4i2.9

Keywords:

Kebo Rubber Leaves, Subfraction, Antibacterial

Abstract

This study aims to determine the antibacterial activity of kebo rubber leaves (Ficus elastica Roxb ex. Hornem) extracts against E. coli and identify the chemical content of the most active subfraction. The leaves were macerated with 70% ethanol and fractionated using n-hexane, ethyl acetate, and water. Subfractions were obtained via vacuum liquid chromatography (VLC) and tested for antibacterial activity using the diffusion method. The ethyl acetate fraction showed the highest antibacterial activity (19.30 mm zone of inhibition). Subfraction 3, with an inhibition zone of 18.73 mm, was the most effective, containing flavonoid compounds with a total flavonoid value of 79.8950 mgQE/g. TLC analysis confirmed the presence of flavonoids and tannins.

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